I put the front ISO back in and removed the head steady completely, the head shifted to the right.
So my engine was tilted 1/8" to the left at the cylinder head
Now with the existing ISO set up and no head steady, the engine is straight in the frame but on the centreline. The rear wheel looks reasonable central in the loop.
When I get the adjusters/abutments from RGM I can work out what has happened.
I am in the process of designing a link type head steady, this should have the adjustability to align the head left/right accurately